Table 1. Summary of in vitro and in vivo CEACAM expression of all cell lines by different methods.
| cell culture | xenografted tumor | ||||
| QPCR | WB | FACS positive | immunocytochemistry | immunohistochemistry | |
| 5061 | +++ | + | 99% | +++ (95%) | ++/+++ (45%) |
| 5072 | ++ | + | 77% | +++ (90%) | − (0%) |
| LNCAP | ++ | + | 93% | ++ (100%) | ++/+++ (60%) |
| PC3 | + | + | 23% | ++ (10%) | (+) (20%) |
| T47D | + | (+) | 5% | + (1%) | +/++ (20%) |
| MCF7 | + | + | 23% | +++ (5%) | +/++ (20%) |
| MEWO | (+) | + | 74% | +++ (85%) | ++ (60%) |
| FemX-1 | + | + | 95% | +++ (100%) | ++/+++ (90%) |
| HT29 | ++ | + | 86% | +++ (90%) | ++ (45%) |
| Caco2 | + | + | 16% | +++ (90%) | ++ (20%) |
| SW480 | − | + | 31% | + (5%) | (+) (30%) |
| OH-1 | (+) | + | 80% | ++ (80%) | (+) (100%) |
Note that the highest CEACAM expression in vitro and in vivo showed the melanoma cell line FemX-1. The intensity (plus signs) and extent (percent) of the positive areas of 5 histological sections were determined by visual inspection of 3 independent observers.