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. 2011 Dec 5;6(12):e28590. doi: 10.1371/journal.pone.0028590

Figure 6. Continuous-flow biofilm formation is dependent on radical- and RecA-induced activation of yneA.

Figure 6

Activation of SOS response member yneA is required to obtain fully grown continuous-flow biofilms that are composed of micro-colonies surrounded by knitted chains composed of elongated cells. A) The graph presents the average and standard deviation of the continuous-flow biofilm produced by the wild-type strain, the ΔrecA mutant, and the ΔyneA mutant grown in BHI, and the wild-type strain grown in BHI with 0.05 mM bipyridyl and 50 mM thiourea using three biological independent experiments. *Data points significantly different from the wild-type strain grown in BHI (p<0.05, t-test). B) The micrographs present phase contrast pictures of continuous-flow biofilms of the wild-type strain grown in BHI without (1) and with 0.05 mM bipyridyl and 50 mM thiourea (2), and of the ΔrecA mutant (3), and the ΔyneA mutant (4) grown in BHI.