Figure 6. GV L protein inhibits transcription from IFN-responsive promoters.

(a) Vero cells were transfected essentially as for Figure 5 except that 100 ng of the reporter gene plasmid pGL3Mx-1-lucwas used. All transfections were made up to the same amount of DNA using empty vector where required. After 40 hours of transfection cells were incubated with or without IFNα. After a further 8 hours the cells were lysed and the luciferase and β-galactosidase activities determined as described in material and methods. (b) Vero cells were transfected essentially as for Figure 5 except that 400 ng of the reporter gene plasmid pGAS-luc was used. 40 hours post-transfection cells were treated with IFNγ. After a further 6 hours cells were lysed and the luciferase and β-galactosidase activities determined. Results from separate experiments were combined by setting the RLUs induced by IFNα in cells transfected with empty vector to 100%. Error bars show standard errors of normalised data.