Figure 4. Adv expressed IK17-scFv immunostains atherosclerotic lesion.
Pooled plasma (1:20 dilution) from Adv-IK17-scFv mice (A) or control Adv-EGFP mice (B) were used to immunostain human brain artery atherosclerotic lesion. Plasma samples were obtained from respective mice 5 days post injection. (C and D) demonstrate that the Adenoviral expressed IK17-scFv localizes to atherosclerotic lesions in the cholesterol-fed LDLR−/−/Rag1−/− mice. Cross-sections at the aortic valve obtained from mouse treated with Adv-IK17-scFv (C) or with Adv-EGFP (D). Sections were obtained at time of sacrifice and immunostained with biotinylated-anti-c-myc.