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Eight-day old neuron cultures were pre-treated with PEDF (100 ng/ml, 24 h) with or without ERK 1/2 kinase inhibitor (5 μM) and exposed to H2O2 (50 μM) for 24 h. Bcl-2 was determined by western blot.
**p<0.01 vs. control; ap<0.01 vs. H2O2; bp<0.01 vs. PEDF;