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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Hear Res. 2011 Aug 12;282(1-2):196–203. doi: 10.1016/j.heares.2011.08.002

Fig. 3.

Fig. 3

Low, but not high concentrations of cisplatin destroyed spiral ganglion neurons (SGNs) and auditory nerve fibers (ANFs). (A-C) Representative confocal photomicrographs of control and cisplatin-treated (50 and 1000 μM) cochlear organotypic cultures showing green-labeled (Alexa Fluor 488-phalloidin) hair cells (upper three rows= OHCs; bottom row= IHCs), and red-labeled (anti-neurofilament 200 antibody) ANFs and SGNs. (B) Following 48 h exposure to 50 μM cisplatin, there was considerable damage to the ANFs and SGNs. (C) In contrast, no damage was observed after exposure to 1000 μM cisplatin. Scale bar shown in panel A represents 10 μm.