Representative in situ hybridization autoradiograms showing Fz6 (A), Slc6a9 (B) and Per1 (C) mRNA in various regions of the rat brain. Coronal section of brain from sham (left) or Chr-ECS-treated rat (right). Data represent mean ± SEM of fold change in mRNA, n=5 per group, and are presented as fold change relative to control (value of 1 indicates no change in expression over control). * p<0.05 (t-test). (D) The influence of chronic unpredictable stress (CUS) on levels of Fz6, Slc6a9 and Per1 mRNA in hippocampus was determined by RT-PCR. Data represent mean ± SEM of fold change in mRNA, n=6 per group. Student’s t-test; * p<0.05 compared to non stressed controls.