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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Biol Psychiatry. 2011 Sep 19;71(1):51–58. doi: 10.1016/j.biopsych.2011.08.004

Figure 5. Experimental outline and Influence of Fz6 shRNA knockdown in models of depression.

Figure 5

(A) Schematic diagram showing details of experimental timeline. (B) AAV-GFP-Scr (Control) or AAV-GFP-Fz6shRNA was infused into the hippocampus. Representative image showing expression of GFP in the hippocampus (inlet, dentate gyrus) of animal infused with AAV-GFP-Fz6shRNA. (C) Data represent mean ± SEM of percent of Fz6mRNA expression relative to AAV scrshRNA at 2 weeks post injection (n=2). Rats were injected with AAVFz6shRNA on one side and AAV-scrshRNA on the other side of the brain. Primary cortical neuronal cultures were incubated with AAV-scrshRNA and 2 weeks post infection levels of Fz6 mRNA were determined. Results are the mean ± SEM of percent of Fz6mRNA expression relative AAV-scrshRNA controls (n=2). The influence of AAV-Fz6shRNA expression on different behavioral models was examined, including sucrose (D), total consumption (E) and sucrose preference (F) in the sucrose preference (SPT) test, # of open arm (G), closed arm (H) entries and time spent in open arms (I) in elevated plus maze (EPM) task, latency to feed in the novelty suppressed feeding (NSF) (J), and escape failures (K) and latency to escape (L) in the learned helplessness-active avoidance test. Results are the mean ± SEM of AAV-scr (n=8) vs. AAV-Fz6shRNA (n=8). ns=not significant; *p < 0.05 compared to AAV-scr (Student’s t-test); #=not significant but significant trend observed at one measure (one-way ANOVA).