Fig. 2.

Depletion of MVI affects Golgi and cytoskeleton organization. MVI distribution in T-MVI 72 h after transfection (a) and SA-MVI cells (b) as well as in control cells (mock control and sc₁MVI or sc₂MVI) was visualized with anti-porcine MVI antibody (green). Golgi structures were stained with anti-GM130 or TGN38 antibodies (in red), microfilaments with TRITC-phalloidin (red) and nuclei with ToPro3 (blue). In a, arrowheads point to the most altered Golgi complexes. These are the 0.8-μm images of the cell center attained with Leica TCS SP2 or SP5 spectral confocal microscopes equipped with HCX PL APO 63x/1.25-0.75 Oil Cs objectives. Other details as described under “Materials and methods” section. Bars 10 μm