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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Neurochem. 2011 Nov 3;119(6):1205–1216. doi: 10.1111/j.1471-4159.2011.07528.x

Figure 4.

Figure 4

Expression of TrkB and p75 NTR in cortical neurons at different DIV. (A) BDNF responses are mediated by TrkB but not p75 NTR. Cultures at DIV 7 (A) or DIV 13 (B) were pretreated with K252a (200nM) or TAT-PEP5 (1 μM) for 30 min before BDNF treatment, then p-ERK1/2 was examined by Western blot (n = 3 for all groups). (C) and (D) Equal amount of protein (60 μg) from neurons at DIV 3, 7, 13, and 22 was separated by SDS-PAGE. The expression level of p75 NTR (C) and TrkB (D) was determined by Western blot (n=3 for each group). The upper panels are representative Western blot results. β-actin was used as an internal control. The lower panels show quantification for the relative expression of p75 NTR and TrkB after normalization to β-actin. One-way analysis of variance (ANOVA) revealed that TrkB expression is statistically different (p < 0.001) in neurons at different DIV. Different letters indicate distinct SNK mean groupings (a > b > c > d). C: control; B: BDNF; B + TAT-PEP5: pretreatment with TAT-PEP5 followed by BDNF stimulation; B + K252a: pretreatment with K252a followed by BDNF stimulation.