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The Cyp3a11 reporter gene (tk-Cyp3a11) was transfected into HepG2 cells together with PXR and/or LXRα expression vectors. Transfected cells were treated with PCN or GW3965 (10 μM each) for 24 h before luciferase assay. The transfection efficiency was normalized against the β-gal activity from the co-transfected CMX-β gal vector.
