Skip to main content
. 2011 Dec;134(4):434–447. doi: 10.1111/j.1365-2567.2011.03502.x

Figure 8.

Figure 8

Absence of MHC class I molecules and mature CD8+ T cells and/or endogenous T-cell receptor (TCR) α-chains does not affect the presence of double-negative (DN) T cells in the periphery. (a) Splenocytes from hepatitis B virus e antigen (HBeAg) × 7/16-5 dbl-Tg mice and MHC class I knockout (KO) × HBeAg × 7/16-5 mice were cultured in the presence of HBeAg-derived peptide p120–140 for 4 days. Cells were collected and analysed for class I, CD8 and Vβ11 expression from the DN gated population by FACS. (b) Splenocytes from TCR α-chain KO × HBeAg × 7/16-5 mice and TCR α-chain KO × 7/16-5 and HBeAg × 7/16-5 mice were cultured in the presence of HBeAg-derived peptide p120–140 for 4 days. Cells were collected and analysed for Vβ11 from the DN gated population by FACS.