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Double-negative (DN) T cells express high levels of ectoenzyme CD73 and CD39 4 × 105 splenocytes/well from hepatitis B virus e antigen (HBeAg) × 7/16-5 were cultured in 96-well plates for 72 hr with 2 μg/ml of HBeAg-derived peptide 120–140, and cells were harvested and analysed for surface expression of CD73 and CD39 by FACS.
