Skip to main content
. 2011 Dec;134(4):448–458. doi: 10.1111/j.1365-2567.2011.03503.x

Figure 5.

Figure 5

Cytokine production by γδ and αβ T cells derived from the intestinal intraepithelial T lymphocytes (IEL) of uninfected wild-type (WT) and T-cell receptor-δ-deficient (TCR-δ−/−) mice. (a) IEL bearing T-cell receptor-αβ (TCR-αβ) and TCR-γδ of naive WT mice were purified by magnetic antibody cell sorting and IEL of TCR-δ−/− mice, which consisted of TCR-αβ alone, was used unseparated. The IEL were treated with anti-CD3 monoclonal antibody (mAb) for 3 days and cytokine production was analysed by ELISA for interferon-γ (IFN-γ), interleukin-4 (IL-4), IL-5 and IL-13. Values represent the mean + SEM of three independent experiments. *P< 0·05. (b) IL-13 mRNA expression in the IEL of uninfected WT and TCR-δ−/− mice. The IEL were treated with anti-CD3 mAb for 6 hr and mRNA expression level of IL-13 was detected by quantitative RT-PCR. The data are normalized to 18s mRNA content and plotted as mean fold over IEL in TCR-δ−/− mice in three independent experiments. (c) IL-13 production in the IEL of uninfected WT and TCR-δ−/− mice by Western blot analysis.