Figure 7.

Expression of an anti-DNA heavy chain transgene in dnRAG1 mice blocks B1-like B-cell accumulation, impairs B-cell development at the transitional T1 stage, and promotes marginal zone (MZ) B-cell differentiation. (a) Lymphocyte-gated cells prepared from wild-type (WT), dnRAG1, 56Rki, and double-transgenic (DTG) spleen (SPL) and peritoneal cavity (PerC) were analysed for the expression of CD19 and B220 as in Fig. 1(c). The absolute number of cells in each population is shown at right (***P <0·005). (b) Cells prepared from WT, dnRAG1, 56Rki, and DTG bone marrow or spleen and identified by the gating parameters shown above each row were analysed as in Fig. 2. (c) The absolute number of cells within the identified gates is shown as in Fig. 2. Significant differences are shown only for comparisons between dnRAG1 or 56Rki mice and DTG mice. See Table S2 for summary.