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. 2011 Nov 2;3(11):2146–2159. doi: 10.3390/v3112146

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Requirement of different HTLV-1 Tax domains for HERV LTR activation. (A,B) Jurkat cells were co-transfected with a reporter plasmid carrying the HERV-W8 (A) or HERV-H (B) LTR and expression vectors for wild type or mutated version of Tax (vs. the empty vector). At 24 hr post-transfection, cells were harvested and measured for luciferase activity. (C) Using an anti-Tax antibody, Western blot analyses was carried out on extracts from Jurkat cells transfected with different Tax mutants to show equal expression of all Tax mutants and Tax WT. GAPDH is shown as a loading control (D) Jurkat cells were co-transfected with the luciferase reporter construct under the control of the HERV-W8 LTR in the presence or absence of a Tax expression vector (vs. the empty vector) and the expression vector for KCREB (vs. empty vector). All transfections were conducted in the presence of pRcActin-LacZ for normalization. Results are shown as normalized luciferase values and represent the mean values of three independently transfected cell samples. A western blot analysis using an anti-Tax antibody was carried out to confirm equal expression of Tax between samples. GAPDH is shown as a loading control.*, P < 0.05;**, P < 0.01.

Figure 3. — Requirement of different HTLV-1 Tax domains for HERV LTR activation. (A,B) Jurkat cells were co-transfected with a reporter plasmid carrying the HERV-W8 (A) or HERV-H (B) LTR and expression vectors for wild type or mutated version of Tax (vs. the empty vector). At 24 hr post-transfection, cells were harvested and measured for luciferase activity. (C) Using an anti-Tax antibody, Western blot analyses was carried out on extracts from Jurkat cells transfected with different Tax mutants to show equal expression of all Tax mutants and Tax WT. GAPDH is shown as a loading control (D) Jurkat cells were co-transfected with the luciferase reporter construct under the control of the HERV-W8 LTR in the presence or absence of a Tax expression vector (vs. the empty vector) and the expression vector for KCREB (vs. empty vector). All transfections were conducted in the presence of pRcActin-LacZ for normalization. Results are shown as normalized luciferase values and represent the mean values of three independently transfected cell samples. A western blot analysis using an anti-Tax antibody was carried out to confirm equal expression of Tax between samples. GAPDH is shown as a loading control.*, P < 0.05;**, P < 0.01.