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. 2011 Nov 14;3(11):2223–2237. doi: 10.3390/v3112223

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — FFV myr-Gag SVP budding is abrogated by the N-myristoyltransferases (NMT) inhibitor 2-OHM. (A) 293T cells were transfected with myr-Gag expression clones (as given above the figures). The NMT inhibitor DL-2-hydroxymyristic acid (2-OHM) was added after the medium change at the end of transfection (about 10 hours after DNA addition) and left on the cells for at least 30 hours. Proteins of cell lysates and particle preparations were harvested and assayed by immunoblotting for FFV Gag. The positions of the FFV p52 Gag protein and β-actin are marked. (B) Densitometric quantification of the relative expression levels and budding capacities of the different FFV myr-Gag proteins in the absence of 2-OHM shows similar intracellular levels of the different FFV Gag myr-sub and myr-add clones (white and black bars, resp.) while the budding capacity of the different FFV Gag myr-add proteins was significantly higher than that of the myr-sub Gag proteins.

Figure 3. — FFV myr-Gag SVP budding is abrogated by the N-myristoyltransferases (NMT) inhibitor 2-OHM. (A) 293T cells were transfected with myr-Gag expression clones (as given above the figures). The NMT inhibitor DL-2-hydroxymyristic acid (2-OHM) was added after the medium change at the end of transfection (about 10 hours after DNA addition) and left on the cells for at least 30 hours. Proteins of cell lysates and particle preparations were harvested and assayed by immunoblotting for FFV Gag. The positions of the FFV p52 Gag protein and β-actin are marked. (B) Densitometric quantification of the relative expression levels and budding capacities of the different FFV myr-Gag proteins in the absence of 2-OHM shows similar intracellular levels of the different FFV Gag myr-sub and myr-add clones (white and black bars, resp.) while the budding capacity of the different FFV Gag myr-add proteins was significantly higher than that of the myr-sub Gag proteins.