Figure 5.

Subcellular distribution of WT and myr-Gag proteins as determined by indirect immunofluorescence (IIF) confocal laser microscopy of transfected and fixed HeLa cells. HeLa cells were transfected with plasmids directing expression of myr-add (A), myr-sub (B), and WT, unmodified Gag (C). Cells were fixed with para-formaldehyde and reacted with a guinea pig antiserum against the FFV Gag capsid domain (red staining) while nuclei were counter-stained with DAPI (blue staining) as described in the Experimental Section. Composite z stacks of two confocal sections (out of 25) are shown. Single confocal sections were acquired at the center of the cells (upper panels) and at the apical, top part of the cells (lower panels).