Figure 7. Distinct ensembles of ChR2-expressing piriform neurons can be entrained to elicit different behaviors.
A. A schematic of the apparatus used for the conditioning paradigm. Initially, stimulation of one ensemble (CS1) was paired with shock on the side of the arena where the animal received the photostimulation, and stimulation of the second ensemble (CS2) was paired with shock to the opposite side of the arena. For a reversal-learning paradigm, the shock contingency was switched between the two ensembles (Reversal Learning).
B. The percentage of trials in which animals exhibited flight behavior in response to CS1 and CS2 after training with the CS-shock contingencies described in A, left (hSynapsin1: N=1, Dual Virus: N=4).
C. The percentage of trials in which flight behavior was elicited by the CS1 and CS2 for a subset of animals shown in B after they were subsequently trained with reversed CS-shock contingencies described in A, right (Reversal Learning, hSynapsin1: N=1, Dual Virus: N=2).