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. 2010 Nov 2;10(11):9831–9846. doi: 10.3390/s101109831

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© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/.)

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Figure 1. — Schematic Protocol for splashing the metaphase spreads followed by rapid assembly of the microFISH device. (a) Bonding of the double-sided adhesive tape stencil on the glass slide; (b) Spreading of the metaphase spreads in the splashing device; (c) Peeling off the top cover of the double-sided tape stencil to leave only the spreads in the centre of the glass slide and to expose the adhesive layer to bond the PDMS microFISH lid; (d) Aligning the PDMS lid on to the adhesive tape; (e) Assembly of the microFISH by bonding the PDMS lid on to the tape using gentle pressure; (f) Making the interconnection holes and connecting the syringes for world-to-microFISH device fluidic connections.