Table 1.

Amplitude α_i, total fluorescence intensity ${\sum }_{i=1}^3{\alpha }_i$, lifetime τ_i, mean lifetime τ̄, and relative amplitude f_i of the three-exponential model for free and immobilized GOD in absence and in presence of glucose.

	GOD free	GOD free + 1 mM glucose	GOD immobilized	GOD immobilized + 2 mM glucose
α1	0.031 ± 0.004	0.02 ± 0.01	0.08 ± 0.01	0.037 ± 0.006
α2	0.035 ± 0.004	0.230 ± 0.003	0.094 ± 0.01	0.476 ± 0.006
α3	0.490 ± 0.003	0.790 ± 0.003	0.4 ± 0.01	0.643 ± 0.005
∑ αi	0.556	1.04	0.574	1.156
τ1 (ps)	3 ± 1	2 ± 1	8 ± 3	4 ± 2
τ2 (ps)	550 ± 60	401 ± 8	380 ± 70	450 ± 7
τ3 (ps)	3, 120 ± 40	3, 061 ± 28	2, 960 ± 190	3, 010 ± 50
τ̄ (ps)	2,784	2,413	2,126	1,860
f1	0.054	0.015	0.14	0.029
f2	0.06	0.22	0.16	0.41
f3	0.88	0.76	0.70	0.56