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. 2012 Jan 1;139(1):33–45. doi: 10.1242/dev.071761

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Fig. 5. — Ptf1a-mediated Dll1 expression is required for MPC proliferation. (A-F) Image stack projections of E10.5 embryos from crosses of Dll1^lacZ^/+Neurog3^tTA/+ double-heterozygote animals, whole-mount stained for Pdx1, Ptf1a and glucagon. Note that dorsal and ventral bud size is equally reduced in Dll1^lacZ^/^lacZNeurog3^+/+ and Dll1^lacZ^/^lacZNeurog3^tTA/tTA embryos. dp, dorsal pancreas; vp, ventral pancreas. Scale bars: 50 μm. (G) Quantification of dorsal and ventral bud volume in wild-type and mutant embryos of the indicated genotypes based on Pdx1/Ptf1a double whole-mount stained and confocally scanned embryos. (H,I) Quantification of BrdU incorporation in E10.5 Pdx1⁺ MPCs in Dll1 and Hes1 mutant dorsal buds, respectively. Data in G-I are represented as mean ± s.d. ##, P<0.0002; , P<0.0005; #, P<0.002; , P<0.005; ^**, P<0.01; ^*, P<0.05; by Student’s t-test (compared with wild type unless otherwise indicated); n=2-4. ns, not significant.

Inline graphic — Ptf1a-mediated Dll1 expression is required for MPC proliferation. (A-F) Image stack projections of E10.5 embryos from crosses of Dll1^lacZ^/+Neurog3^tTA/+ double-heterozygote animals, whole-mount stained for Pdx1, Ptf1a and glucagon. Note that dorsal and ventral bud size is equally reduced in Dll1^lacZ^/^lacZNeurog3^+/+ and Dll1^lacZ^/^lacZNeurog3^tTA/tTA embryos. dp, dorsal pancreas; vp, ventral pancreas. Scale bars: 50 μm. (G) Quantification of dorsal and ventral bud volume in wild-type and mutant embryos of the indicated genotypes based on Pdx1/Ptf1a double whole-mount stained and confocally scanned embryos. (H,I) Quantification of BrdU incorporation in E10.5 Pdx1⁺ MPCs in Dll1 and Hes1 mutant dorsal buds, respectively. Data in G-I are represented as mean ± s.d. ##, P<0.0002; , P<0.0005; #, P<0.002; , P<0.005; ^**, P<0.01; ^*, P<0.05; by Student’s t-test (compared with wild type unless otherwise indicated); n=2-4. ns, not significant.