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miR-29 protects against CKD-induced suppression of MPCs differentiation. MPCs isolated from hind-limb muscles of CKD or sham-operated, pair-fed mice (sham) were cultured in differentiation media on Entactin, Collagen IV, and Laminin matrix coated plates. Immunohistochemistry with an antibody against eMyHC was used to assess MPCs differentiation into myotubes. Nuclei (blue) were stained with 4-diamidino-2-phenylindole, and red identifies eMyHC.
