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. 2010 Jun;24(6):1925–1934. doi: 10.1096/fj.09-150573

Figure 1.

Figure 1

TSP1 deficiency impairs NPC proliferation and neuronal differentiation in vivo. A–H) To examine proliferation of neural progenitors in TSP1−/− and WT mice, exogenous (BrdU; A–D) and endogenous (Ki-67; E–H) proliferation markers were used. Labeled cells were quantified in SGL (A, B, E, F) and SVZ (C, D, G, H) areas of the brain. A significant reduction in proliferating cells was found in both SVZ and SGL of TSP1−/− mice compared to WT counterparts. I–L) To examine neuronal differentiation in TSP1−/− and WT mice, brain slices containing SGL (I, J) and SVZ (K, L) were double-labeled with DCX and BrdU at 7 d after BrdU injection. Significantly less DCX/BrdU double-positive cells were found in TSP1−/− as compared to WT mice. Quantification data are from ≥4 animals/group. Confocal micrographs are representative. ***P < 0.001; Student’s t test. Scale bars = 50 μm (B, D, F, H, L); 20 μm (J).