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. 2010 Jun;24(6):1925–1934. doi: 10.1096/fj.09-150573

Figure 5.

Figure 5

TSP1 is required for astrocyte-induced neuronal differentiation. A–D) P4 NPCs from TSP1−/− (A, B) and WT (C, D) mice were cultured for 7 d in the presence of ACM obtained from either TSP1−/− or WT astrocytes. Control cells were kept in regular differentiation medium. Incubation of TSP1−/−-derived NPCs with WT-ACM increased neuronal (A) and decreased oligodendrocyte (B) differentiation. TSP1−/−-ACM did not affect differentiation of TSP1−/− NPCs. Incubation of WT NPCs with WT-ACM resulted in increased neuronal (C) and decreased oligodendrocyte (D) differentiation. TSP1−/−-ACM did not affect differentiation of WT NPCs. E) Representative micrographs of WT and TSP1−/− NPCs differentiated under different conditions.. Data are means ± se of ≥3 independent experiments. *P < 0.05; Student’s t test. Scale bar = 10 μm.