Figure 1.

JNK inhibition affects FGF-2/HS– and TGF-β1–induced assembly and organization of actin filaments in activated stromal keratocytes. Corneal stromal keratocytes, isolated from rabbit corneas, were cultured in DMEM/F12 without FBS (SFM). After pretreating with or without SP600125 for 3 hours in the SFM, the keratocytes were activated by supplementing the medium in the culture dishes with 40 ng/mL FGF-2 and 5μg/mL HS or 2 ng/mL TGF-β1. After 60 hours of incubation, the cells were reacted with Alexa Fluor 546-phalloidin. Bar, 50 μm.