Figure 2.

JNK inhibition occludes TGF-β1– and FGF-2–induced decreases in cell surface–associated KS in the activated corneal stromal keratocytes. Corneal stromal keratocytes, isolated from rabbit corneas, were cultured in DMEM/F12 without FBS (SFM). After pretreating with or without SP600125 for 3 hours in the SFM, the keratocytes were activated by supplementing the medium in the culture dishes with: (A) 40 ng/mL FGF-2 and 5 μg /mL HS; or (B) 2 ng/mL of TGF-β1. After 60 hours of incubation, cells were analyzed by double fluorescence staining using mouse anti-KS antibody or anti-p-c-Jun antibody followed by Alexa Fluor 488 anti-mouse IgG antibodies and Alexa Fluor 546 phalloidin. Bar, 50 μm.