Figure 5.

Downegulation of JNK1/2 with JNK1/2 DsiRNA increases levels of lumican and keratocan mRNAs. Keratocytes cultured in DMEM/F12 without FBS (SFM) were first transfected with JNK1/2 DsiRNAs or scrambled DsiRNA, and then either activated with 40 ng/mL FGF-2 and 5 μg/mL HS or TGF-β1 (2 ng/mL) or not activated (SF transfected controls) and incubated for 60 hours. JNK1, JNK2, lumican, and keratocan mRNA levels were then analyzed by real time RT-PCR using levels of 18S rRNA to normalize values in each experiment. The values presented in the bar graph are the mean ± SD from three different experiments (*P < 0.05).