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RPCs protect RECs from activated T cell–induced apoptosis. Mouse RECs were cultured in wells of a 24-well plate (5 × 105 per well), together with a culture insert containing 4 × 106 of anti-CD3–activated T cells and different numbers of RPCs. RPCs cultured alone and cultured with quiescent T cells were included as controls. In 48 hours, apoptosis of the RECs was assessed by apoptosis detection kit staining. Representative results of two individual experiments are shown.
