Figure 5.
Expression of HPV-L1 and GFP transcripts over time using real-time PCR in restrictive mammalian cells. After infecting Vero cells with 2 PFU/cell of the FPL1 recombinant, quantitative expression of the HPV-L1 and GFP transgenes was evaluated every 3 days for 22 days. The maximum expression level for both HPV-L1 and GFP were reached on day 7 p.i., and GFP mRNAs were always highly expressed. The copy number of HPV-L1/GFP mRNAs, normalised to the RPS7 endogenous housekeeping transcripts, refers to the N-fold increase vs control mRNA at each time point. The data are expressed as means (±standard deviation) from two independent analyses.