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. 2010 May;24(5):1552–1571. doi: 10.1096/fj.08-125856

Figure 8.

Figure 8

Gene expressions were up- or down-regulated in anti-miRNA-treated RPE. qRT-PCR for different genes was performed using cells treated with anti-miR-204 or anti-miR-211. Anti-miRNA derived from C. elegans and anti-miR-222 are controls that have no significant effect on TER A) Transcription factors Jun, SNAIL1, SNAIL2, Smad3, and cingulin (CGN) mRNA were assayed in anti-miR-204- or anti-miR-211-treated cells (n=6). B) Smad4, TGF-βR2 (TGFBR2), IGFR2, IGFBP3, CXCL12, and platelet-derived growth factor B (PDG) expression in anti-miR-204- or anti-miR-211-treated cells (n=4). C) SLC4A4, PCDH18, LRAT, TTR, and RPE65 are down-regulated in anti-miR-211- or anti-miR-204-treated cells (n=6). D) Claudin 10 and 19 expression in anti-miR-204-treated cells. Anti-miR-222 is a control (n=4). E) TTR secretion assayed by ELISA in cell culture medium. Cells were transfected with anti-miR-204/211 for 6 d. Fresh medium was added at d 6, and samples were collected 3 d later for ELISA. For all panels, data are means ± se from triplicates vs. anti-miR control. *P < 0.05; **P < 0.01; #P < 0.005; ##P < 0.001.