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. 2011 Oct 20;25(12):2144–2156. doi: 10.1210/me.2011-0101

Fig. 1.

Fig. 1.

Down-regulation of IGF-II/M6P receptor expression by RNA interference inhibits IGF-II-dependent activation of SK1. A, HEK293 cells were transfected with control scrambled siRNA (SCR) or siRNA targeting the IGF-II/M6P receptor (siIGF-2R) for 48 h. The levels of IGF-II/M6P receptor and GAPDH were determined by immunoblotting whole-cell lysates. A representative IGF-II/M6P receptor and basal GAPDH immunoblots are shown above a bar graph depicting mean ± sd for three independent experiments. B, RNA was isolated and mRNA levels of IGF-II/M6P receptor and GAPDH were determined by quantitative real-time PCR. C, Serum-starved cells transfected with scrambled siRNA or siRNA targeting the IGF-II/M6P receptor were stimulated with 10 nm IGF-II or 100 nm PMA for 10 min after which SK1 activity in whole-cell lysates was assayed as described. Data shown represent the mean ± sd of three independent experiments. *, P < 0.05 vs. nonstimulated (NS); #, P < 0.05 vs. scrambled (SCR) treated.