Fig. 3.

IGF-II stimulation causes rapid and transient translocation of GFP-PKCβ2 to the plasma membrane. Serum-starved HEK293 cells transiently transfected with GFP-PKCα, β1, β2, δ, and ζ were stimulated with 10 nm IGF-II or 100 nm PMA for (A) 2 min (panel A) or at time indicated (panel B) before fixation and examination of the cellular distribution of GFP-PKC by confocal fluorescence microscopy. Shown are representative confocal fields from one of three independent experiments that gave similar results. Scale bar, 10 μm. NS, Nonstimulated.