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IGF-II induces persistent membrane localization of the GFP-PKCβ2(K371R) mutant. Serum-deprived HEK293 cells transiently transfected with GFP-PKCβ2(K371R) were stimulated with 10 nm IGF-II for the indicated time before fixation and examination by confocal fluorescence microscopy. Shown are representative confocal images from one of three independent experiments that gave similar results. Scale bar, 10 μm. NS, Nonstimulated.
