Fig. 7.

PGC-1α S589_4A mutant has increased stability and transcriptional function. A, Half-life of wild type (WT) (solid) and S589_4A mutant (dashed) PGC-1α. HEK293 cells transiently transfected with WT or S589_4A mutant PGC-1α were treated with cycloheximide (CHX) for indicated time points. Protein abundance was quantified and normalized to tubulin for the calculation of half-life. B and C, Luciferase reporter gene assay using PEPCK (B) and G6Pase (C) in the presence of WT or S589_4A PGC-1α in transiently transfected HepG2 cells. Data represent mean ± sd of one representative experiment performed in triplicate. *, P < 0.05.