Fig. 2.

MLL3 complex is a coactivator of p53 in regulating SHP expression. A, ChIP analysis of HepG2 cells treated with vehicle or 0.5 μm DXR for 24 h to examine establishment of active chromatin and recruitment of the MLL3 complex to SHP-p53REs. B, ChIP analysis of HepG2 cells treated with 0.5 μm DXR for 24 h, followed by second ChIP of p53-associated chromatin by IgG and p53 and MLL3 antibodies to examine coassociation of p53 and MLL3 with SHP-p53REs. All experiments were repeated more than three times with similar results.