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. 2011 Nov 24;52(12):9084–9090. doi: 10.1167/iovs.11-8632

Figure 1.

Figure 1.

Chromatographic detection of A2-GPE. Representative reversed-phase UPLC chromatograms with 430 nm and mass monitoring. (A) Profile generated when injectant was a hydrophobic extract of whole Abca4−/− mice eyes, age 4 months; 4 eye cups were pooled. Top insets, UV-visible absorbance spectra of A2E, isoA2E, A2-GPE. (B) Selected ion chromatogram at m/z 746 with retention times corresponding to peaks 3, 4, 5, and 6 in (A). (C) Extract of RPE/choroid isolated from Abca4+/+ mice; age 4 months, 14 eyes pooled. Chromatogram (430 nm absorbance monitoring) expanded between retention times 14.5–25 minutes. Peaks 1 to 6 correspond to the same numbered peaks in (A). (D) UPLC quantitation of RPE lipofuscin pigments A2E and A2-GPE in eyecups of Abca4−/− and Abca4+/+ mice as a function of age. Mean ± SEM; two to three samples; 4 eye cups per sample.