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Hydrolysis of A2-GPE by PLD yields A2E. (A) A2-GPE without incubation. (B) A2-GPE sample after incubation with PLD. Starting compounds (A2-GPE) and cleavage products were detected by reversed phase UPLC-ESI-MS column (Waters XBridge) and monitoring at 430 nm (gradient A). Insets: UV-visible absorbance and selected ion monitoring of the indicated compounds. PLD cleaves at the phosphodiester bond in A2-GPE to release A2E.
