Fig. 5.

CUR-regulated Neurog1 and chromatin remodeling proteins expression. Control and CUR-treated cells were harvested using a RIPA buffer with protein inhibitor cocktail (Sigma); the protein concentrations of the cleared lysates were determined using the BCA method and 20 μg of total proteins each was resolved by 4–15% SDS-polyacrylamide gel electrophoresis, followed by immunoblotting with different antibodies. Neurog1 and methyl-binding proteins and DNMTs were shown in a followed by the image quantification with ImageJ (NIH) and normalized using anti-actin b; HDACs (1–5,8) are shown and quantified in c. The different sources of the antibodies are described in “Experimental Section”