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CUR treatment decreased total HDAC activity. Four-microgram nuclear extracts were prepared from LNCaP cells treated with DMSO, 5 μM CUR, 5 μM CUR with 2.5 μM 5-Aza, and 5 μM CUR with 500 nM TSA for 7 days (TSA was added 20 h before harvest) using Epiquik Nuclear Extraction Kit (Epigentek, NY, OP-0002-1), and the HDAC activity assay was performed following the manufacturer’s (Epigentek, P-4002) instructions
