Fig. 8.

CUR decreased the enrichment of tri-methylation of histone 3 lys 27 (H3K27me3) at Neurog1 promoter region and global level. a For the LNCaP cells treated with DMSO and CUR for 7 days, ChIP assay was performed to pull down the antibody–chromatin complex using anti-H3K27me3 antibody. The details were described in “Experimental Section” and quantified by Q-PCR. b For LNCaP cells treated with DMSO, CUR, and 5-Aza/TSA (TSA was added 20 h before harvest), global enrichment of H3Lys27me3 was measured by histone H3 methylated lys27 ELISA (Active Motif, Carlsbad, CA) following the manufacturer’s instructions