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. 2011 Aug 30;10(Suppl 1):S16. doi: 10.1186/1475-2859-10-S1-S16

Figure 6.

Figure 6

Transcriptional organization of the bifidobacterial pili loci in B. bifidum PRL2010. (panel a-c) Schematic diagram of bifidobacterial pilus locus. Hairpin symbols indicate predicted secondary structures. Panels d-f, products generated by RT-PCR. PCR products were obtained with primers spanning the intergenic regions between the pilin encoding subunits (primers Pil1_1f/ Pil1_1r, Pil2_2f/Pil2_2r and Pil3_1f/Pil3_1r) (lanes 4-5, 22-24, 29-31), between pilin encoding subunits and the srtA gene (primers Pil1_2f/Pil1_2r, Pil2_1f/Pil2_1r and Pil3_2f/Pil3_2r) (lanes 7-8, 19-21, 32-34), between pilin encoding subunits and ORF1823, ORF1706, ORF281 (primers Pil1_4f/Pil1_4r; Pil2_4f/Pil2_4r and Pil3_3f/Pil3_3r) (lanes 1-3, 16-18, 35-37), and between the srtA gene and ORF 1819, 1710 and 285 (primers Pil1_5f/Pil1_5r; Pil2_3f/Pil2_3r and Pil3_4f/Pil3_4f) (lanes 10-12, 25-27, 38-40). The positions of the primer pairs used in RT-PCR experiments are shown in panels a-c. Products of the PCR were derived under the following conditions: cDNA prepared from B. bifidum PRL2010 RNA cultivated on MRS supplemented with bovine milk and/or FOS (lanes 1, 4, 7, 10, 13, 16, 19, 22, 25, 29, 32, 35, 38). A positive control control B. bifidum PRL2010 DNA (lanes 2, 5, 8, 11, 14, 17, 20, 23, 26, 30, 33, 36, 39). A negative control in which B. bifidum PRL2010 RNA was used but reverse transcriptase was omitted (lanes 3, 6, 9, 12, 15, 18, 21, 24, 27, 31, 34, 37, 40). Lanes MK contained DNA molecular marker 1kb