Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli JM109 | Cloning host for pGEMTeasy- and pJRS233-derivative plasmids | Promega |
| Lactobacillus reuteri 100-23 | Rodent isolate; wild type strain | [25] |
| ΔgadB | Wild-type strain derivative with a deletion in gadB | This study |
| Plasmids | ||
| pGEMTeasy | Cloning vector used in E. coli; 3.0 kb; Ampr | Promega |
| pGadB-A | pGEMTeasy containing 1 kb of the DNA sequence upstream of gadB; 4.0 kb; Ampr | This study |
| pGadB-B | pGEMTeasy containing 0.9 kb of the DNA sequence downstream of gadB; 3.9 kb; Ampr | This study |
| pGadB-AB | pGEMTeasy containing the upstream and downstream sequences of gadB; 4.9 kb; Ampr | This study |
| pJRS233 | Shuttle vector used in the hosts E. coli and L. reuteri 100-23; 6.0 kb; Ermr | [29] |
| pKO-gadB-AB | pJRS233 containing 1.9 kb of the flanking sequences of gadB; 7.9 kb; Ermr | This study |
Ampr: amplicillin-resistance gene; Ermr: erythromycin-resistance gene