Figure 4.
Conceptual framework for measuring and correlating mild stress induced biomarker and robustness responses and evaluating the predictive potential of biomarkers. A) Candidate-biomarkers – σB (activating general stress responses), catalases (removing reactive oxygen species, ROS), and chaperones and proteases (maintenance of protein quality) – were quantitatively measured at transcript, protein and/or activity level upon mild stress treatment. Mild stressed cells were also exposed to lethal stress to quantify their robustness advantage compared to unstressed cells. B) To evaluate the predictive potential of the candidate-biomarker, the mild stress induced biomarker responses were correlated to the robustness advantage of mild stressed cells, and the correlation was tested for significance (figure is adapted from Besten et al. [53]).