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The MBV-R mutants pUL27(A269T) and pUL27(R233S) are excluded from nucleoli throughout HCMV infection. HFFs were infected with T3607, T3614, T3654, and T3675 (Table 2). Indirect immunofluorescence for nucleolin was performed at 20 hpi (A) and 96 hpi (B), and the localization of each pUL27-EGFP construct and nucleolin was determined by fluorescence microscopy.
