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. Author manuscript; available in PMC: 2011 Dec 6.
Published in final edited form as: J Cereb Blood Flow Metab. 2007 Sep 26;28(3):612–620. doi: 10.1038/sj.jcbfm.9600560

Table 1.

Population densities and percent ratios for neurons immunoreactive for either HIF-1α or VEGFR-2 from total neuronal populations

Ml hand (infarct) Peri-infarct PMv hand Ml hindlimb Ml orofacial somatosensory temporal
Control (n = 3)
    HIF-Neurons/mm3 1,274 1,212 1,564 1,078 1682 1558 1119
    Neurons/mm3 37,611 40,426 38,638 42,385 44,115 59,319 59,265
    Percent ratio 3.5 3 4.1 2.4 5.5 2.7 1.9
    Distance (mm) 2.58 5.06 6.91 4.28 5.85 16.55
Infarct (n = 4)
    HIF-Neurons/mm3 7,941 26,394 1,478 2,194 929 1146 464
    Neurons/mm3 8736 29,984 32,649 43,840 37,652 59,856 61,132
    Percent ratio 87.1 89.2 5.9 5 2.5 2.6 0.9
    Distance (mm) 2.61 6.59 6.67 5 5.49 16.41
    F value 65.37 467.86 0.13 7.01 0.34 0.03 4.24
    Significance P < 0.001 P < 0.0001 P > 0.05 P < 0.05 P > 0.05 P > 0.05 P > 0.05
Control (n = 3)
    VEGFR-2-Neurons/mm3 9376 9805 10,291 10,221 12,674 10,477 6509
    Neurons/mm3 49,641 52,237 48,718 56,303 66,958 80,166 58,966
    Percent ratio 18.5 18.7 21.3 18.7 19.3 13 10.7
    Distance (mm) 2.58 5.06 6.91 4.28 5.85 16.55
Infarct (n = 4)
    VEGFR-2-Neurons/mm3 3,215 22,867 19,777 18,344 25,005 20,651 10,858
    Neurons/mm3 6,221 73,423 70,525 72,592 103,549 107,639 80,523
    Percent ratio 53.4 32.1 29 25 24.4 19.5 14.3
    Distance (mm) 2.61 6.59 6.67 5 5.49 16.41
    F value 53.97 12.47 7.68 7.19 4.61 3.66 1.96
    Significance P < 0.001 P < 0.05 P < 0.05 P < 0.05 P > 0.05 P > 0.05 P > 0.05

ANOVA, analysis of variance; HIF, hypoxia-inducible factor; M1, primary motor; PMv, ventral premotor; VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor-2.

Population estimates are shown as total neurons/mm3 and represent the mean (mean ± s.d.) of population numbers from all animals in either experimental or control groups as calculated by Stereo Investigator. Percent ratios represent the amount of neurons that are immunoreactive for HIF-1α protein or VEGFR-2 within the total neuronal population. Significant differences between experimental and control ratios for a given physiologic area are listed and bolded (one-way ANOVA, P < 0.05). Distance (mm) was measured from the center of the M1 hand representation to the center of each of the remaining physiologic areas. Peri-infarct distance represents an additional mean of four distances (0°, 90°, 180°, 270°) as it surrounds the M1 hand representation.