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. Author manuscript; available in PMC: 2012 Oct 20.
Published in final edited form as: Neuron. 2011 Oct 20;72(2):202–230. doi: 10.1016/j.neuron.2011.09.021

Figure 2. Generation of Transactivator Fly Lines.

Figure 2

(A) Transposon enhancer trapping. A transposon containing a minimal promoter and GAL4 is mobilized into the genome through a transposase. Upon insertion in the genome it can be influenced by different enhancers. (B) Plasmid transgenesis. Different enhancers are cloned in front of a minimal promoter and GAL4, and integrated into the same attP docking site with ΦC31integrase, allowing direct comparison of regulatory influences. (C) The MiMIC system. A 5’UTR intronic MiMIC insertion can be converted into transactivator lines (GAL4 or QF) using a gene trap strategy and ΦC31-mediated RMCE. (D) The G-MARET system. A previously generated GAL4 line that is under the influence of an enhancer can be converted into novel ones (QF or LexA) using ΦC31transgenesis. Unwanted sequences flanked by LoxP sites are removed with Cre recombinase. (E) The InSITE system. A previously generated GAL4 line that is under the influence of an enhancer can be converted into novel ones (QF or LexA) using ΦC31transgenesis. Donor constructs contained within transposons can be mobilized in vivo with Flp recombinase. Unwanted sequences flanked by LoxP sites are removed with Cre recombinase. (F) Recombineering. PCR cassettes containing binary factors (QF or GAL4) are recombined into a genomic DNA fragment and the resulting transgene is integrated using ΦC31transgenesis.