Table 1.
Apparent RNA dissociation constants (Kd-values) from filter binding determinations of PyrR binding to the three pyr operon binding loops
| Kd -values (nM) |
|||
|---|---|---|---|
| BcBL1 | BcBL2 | BcBL3 | |
| No effector | 0.87 ± 0.3 | 0.13 ± 0.02 | 0.21 ± 0.08 |
| UMP | 0.07 ± 0.02 | 0.04 ± 0.01 | 0.08 ± 0.05 |
| UDP | 0.07 ± 0.02 | 0.04 ± 0.01 | ND |
| UTP | 0.09 ± 0.02 | 0.04 ± 0.01 | ND |
| dUMP | 0.16 ± 0.08 | 0.05 ± 0.01 | ND |
| PRPP | 0.11 ± 0.03 | 0.06 ± 0.01 | ND |
| GMP | 79 ± 17 | 5.2 ± 2.9 | 49 ± 25 |
| GDP | 37 ± 12 | 2.7 ± 1.6 | ND |
| GTP | 12 ± 3 | 1.1 ± 0.2 | ND |
| dGMP | 9 ± 5 | 0.73 ± 0.01 | ND |
The effectors were present at 0.5 mM. The data are averages of at least three independent determinations and include standard deviations of the mean value. ND, not determined.