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Half-maximum concentrations of nucleotides or PRPP required for either activation (UMP, UTP and PRPP) or inhibition (GMP and GTP) of binding of PyrR to BcBL1 and BcBL2
| Half-maximum concentration (μM) |
||
|---|---|---|
| BcBL1 | BcBL2 | |
| UMP | 0.04 ± 0.02 | 0.02 ± 0.01 |
| UTP | 0.3 ± 0.2 | 2.4 ± 0.7 |
| PRPP | 0.7 ± 0.3 | 2.0 ± 1.4 |
| GMP | 269 ± 143 | 232 ± 162 |
| GTP | 18 ± 6 | 8 ± 6 |
The data are averages of at least two independent determinations.
