Table 3. Effects of the ratio of guanosine to uridine nucleotide concentrations on binding of PyrR to BcBL2.

The total concentration of nucleotides was held constant at 1 mM.

Concentration of nucleotide (μM)		Kd value for RNA (nM)	Concentration of nucleotide (μM)		Kd value for RNA (nM)
GMP	UMP		GTP	UTP
0	0	0.13	0	0	0.13
0	1000	0.06	0	1000	0.05
100	900	0.06	50	950	0.06
250	750	0.05	150	850	0.09
500	500	0.11	250	750	0.11
750	250	0.29	500	500	0.17
900	100	0.84	750	250	0.40
950	50	1.1	900	100	0.61
1000	0	9.8	1000	0	0.75